Merging perfusion, volumetric bioprinting and porous hydrogels to biofabricate a bone marrow model

Abstract

Hematopoietic stem cells (HSCs) hold a distinct and pivotal role as the progenitors of diverse blood cell lineages throughout an individual's lifespan. The sustenance, regulation, and functionality of these vital cells are intricately intertwined with the bone marrow niche. However, the homeostatic equilibrium of the hematopoietic stem cell (HSC) niche is vulnerable to perturbation due to factors such as malignancies or other disruptive influences. The amalgamation of hydrogel matrices and cutting-edge bioprinting techniques represents an advanced strategy in the realm of bone marrow simulation. In our pursuit, we endeavor to establish a more refined in vitro HSC model. This pursuit involves meticulous adjustments to hydrogel solidification conditions and bioprinting parameters, aiming to attain a tailored platform that closely emulates the intricate HSC microenvironment.